Fig. 3.
![Fig. 3. Proliferative responses of HVS-Normal (HVS-N8 [•]) and HVS-WAS (HVS-WAS1 [▵] and HVS-WAS7 [▴]) immortalized T-cell lines after stimulation with immobilized OKT3 MoAb and allospecific Raji cells. Overnight-starved T cells were plated (105 cells/well) in 96-well plates that had been precoated with 10, 25, 50, or 100 ng/well of OKT3 MoAb (left panel) or cocultured in the presence of 25, 50, or 100 × 103 mitomycin-C treated allospecific Raji cells (right panel). After 48 hours of culture, cells were labeled with [3H]-thymidine for an additional 16 hours. Thymidine incorporation was determined by liquid scintillation counting.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/8/10.1182_blood.v90.8.3089/4/bl_0026f3.jpeg?Expires=1724162980&Signature=492pl3vDwHBZ-zo2LHdHZJfGYiz-CMZ9Q~ybv6sukX--W7i-2eSSGOH-0em1o8Jz4wsF4kP1kgQhWFDmSTe7t0aEd7NkPYiW1CDwlsuGg24vJ02YPWqaqppW5oYNXgq1W65cSrj8zCqLsM8TCa9vw9lLKhpZxXXs78Zu4CQeVc6DbjFdfV4w~s6JFa9L~4rQ9ugx-hC5imwEFwNDn4lLLFAolqMBXfqF1K8TX4BwrAVmu7d0dX1U5j8N2Ipu-fOmz0z9XydVklkcaLl0XeTU3AQ0gFS3j4rG7PUh1o-O6qOp~ntLRoaXY5461TFPkWIl0~k0PfxonZ9PhEqfs-9LJQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Proliferative responses of HVS-Normal (HVS-N8 [•]) and HVS-WAS (HVS-WAS1 [▵] and HVS-WAS7 [▴]) immortalized T-cell lines after stimulation with immobilized OKT3 MoAb and allospecific Raji cells. Overnight-starved T cells were plated (105 cells/well) in 96-well plates that had been precoated with 10, 25, 50, or 100 ng/well of OKT3 MoAb (left panel) or cocultured in the presence of 25, 50, or 100 × 103 mitomycin-C treated allospecific Raji cells (right panel). After 48 hours of culture, cells were labeled with [3H]-thymidine for an additional 16 hours. Thymidine incorporation was determined by liquid scintillation counting.
