WASP mRNA expression of HVS-Normal (HVS-N8) and HVS-WAS (HVS-WAS1) immortalized T-cell lines as detected by RT-PCR. Equal amounts of total RNA extracted from indicated cell lines were reverse-transcribed as detailed in the Materials and Methods. cDNAs were amplified by PCR using a pair of primers spanning WASP sequence nucleotides 20-39 (forward) and 980-961 (reverse). Amplification of HVS-Normal cells yields the expected fragment of 900 bp, whereas no equivalent band is detected in the HVS-WAS1 cells. In the last lane, the PCR-negative control (no template) was loaded.