Fig. 3.
![Fig. 3. Blocking of IFN-γ during generation of IL-2/IL-12 LAK cells does not reverse the IL-12-promoting effect on LAK cell lysis of B7-1– or B7-2–expressing tumor cells. The cytolytic activity of AKR-derived IL-2 A-LAKs is compared with the cytolytic activity of untreated or anti–IFN-γ MoAb-treated IL-2/IL-12 A-LAKs on BW-Li, BW-Li(B7-1), and BW-Li(B7-2) targets. The results are represented as the percentage specific [111In]-release (±SE) at different E/T ratios. Standard deviations less than ±3% are not shown for the sake of clarity. Spontaneous release was less than 4%. The experiment was repeated twice. (▧), IL-2 A-LAK; (▧), IL-2 + IL-12 A-LAK; (▩), IL-2 + IL-12 A-LAK + α-IFN-γ.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/1/10.1182_blood.v91.1.196/3/blod4012803.jpeg?Expires=1724147899&Signature=rqaznBzle4hMVNVAqZlWNGymMOsOs7~MENpWPNOdtz4JIrSfxMJdze9~7rZFNeHNibnLMdozZHzD36eTQjcljPE6XvNpmca0EHk0A7L-eXhWotYAsyp6EG7w2RbgSG0udfjE6nGoXx-Ekia7bbgPLHcDK-CZtAmfg7iIWyoEebu-3ruHAC3JRJhGBv5dHucyeRkhL2I57wLUR3~TWrGQ1SCPw88-Zq6un0r0epR4N340YbOwABEiJNCglGrLmbkerB9Psmn2l8EYc7Ucq7guzpJt8DALr4wLGCBv6C4~yqtlUt6-42fFPJO8s290-rgrWrKsptkk-B~gQWYmdFqZfA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Blocking of IFN-γ during generation of IL-2/IL-12 LAK cells does not reverse the IL-12-promoting effect on LAK cell lysis of B7-1– or B7-2–expressing tumor cells. The cytolytic activity of AKR-derived IL-2 A-LAKs is compared with the cytolytic activity of untreated or anti–IFN-γ MoAb-treated IL-2/IL-12 A-LAKs on BW-Li, BW-Li(B7-1), and BW-Li(B7-2) targets. The results are represented as the percentage specific [111In]-release (±SE) at different E/T ratios. Standard deviations less than ±3% are not shown for the sake of clarity. Spontaneous release was less than 4%. The experiment was repeated twice. (▧), IL-2 A-LAK; (▧), IL-2 + IL-12 A-LAK; (▩), IL-2 + IL-12 A-LAK + α-IFN-γ.
