Fig. 7.
Effect of OA on phosphorylation of the hsc70 complex. 32P-labeled resting platelets (5 × 108 platelets/mL) preincubated for 10 minutes at 37°C with either 1 μmol/L OA (OA-treated) or with 0.1% DMSO (control) were lysed and immunoprecipitated with anti-hsc70 MoAb. To avoid gel overexposure after OA treatment, only 25% of the total immunoprecipitated material from control and OA-treated platelets was applied to the 8% SDS-PAGE minigels, dried, and analyzed by autoradiography. One of five similar experiments is presented.
