Fig. 4.
![Fig. 4. In vitro IFN-γ production from lymph node cells isolated during the period of rmIL-12 treatment. Mice received a single injection of either vehicle (A) or rmIL-12 (0.5 μg) (B) 7 days before daily rmIL-12 administration (days 0 to 5). Lymph nodes (popliteal, axillary, and brachial) were harvested during the period of daily rmIL-12 administration for in vitro cytokine assays. The cells were stimulated with either rmIL-12 (0.1 [] or 1 ng/mL [▪]) or Con A (0.5 μg/mL, ▨) as described in the Materials and Methods section. IFN-γ secretion was measured by a commercially available ELISA (Endogen). (▨), Control.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/7/10.1182_blood.v90.7.2541/3/bl_0034f4.jpeg?Expires=1723256469&Signature=nf1FxSO6K2fPP~ZuwYKz68Sx7qMoQOPXr-AsuuGNYzMazyezBZPgHEMoMaGww3-vHnDalA2bumI5xi77FnpptsXPgMum0L4BEH4ylXO8NMfzXzWYRZsZ3eJLZBsUrMCNii7CjqDWt7fU5yE7pGPN2vOUxc5yUZYugf~bpju5icCTtYVwSYuHngDba0jBRwdsfYE8URYNm56ajw8ZoVkQrwDN6aa9hRHg-gLgVGw6cEs~KGSdXuaRDsiiW~6VPcyO9odmrfRxQi2ScLg4k0HDjYaMMV6Cf1X-ZEoVtMDgPUCLa~NuVKpj48PsReVKciLzBhlVVCWqh3kEPxv1NaU3vA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
In vitro IFN-γ production from lymph node cells isolated during the period of rmIL-12 treatment. Mice received a single injection of either vehicle (A) or rmIL-12 (0.5 μg) (B) 7 days before daily rmIL-12 administration (days 0 to 5). Lymph nodes (popliteal, axillary, and brachial) were harvested during the period of daily rmIL-12 administration for in vitro cytokine assays. The cells were stimulated with either rmIL-12 (0.1 [] or 1 ng/mL [▪]) or Con A (0.5 μg/mL, ▨) as described in the Materials and Methods section. IFN-γ secretion was measured by a commercially available ELISA (Endogen). (▨), Control.
