Fig. 5.
FACScan analysis of spleen cells from Swiss nu/nu mice. Swiss nu/nu mice, in groups of 3, were injected IV with live 105 GM-AML or B7.1-AML or mock-infected AML cells. Three days later, their spleen cells were stained with a panel of 20 MoAb as described in the Materials and Methods and compared with spleen cells from naive Swiss nu/nu mice. A total of 10,000 cells were analyzed by FACS for each sample. GM-AML–injected mice showed a higher population of cells negative for B220 (25.31% v 18.51% in naive mice) and increased expression of CD80, CD86, IL-2R, CD18, and Mac-1.
