Only the CD14-derived DCs bind immune complexes. The CD1a- and CD14-derived DC subsets were obtained as described in Materials and Methods and in the legend to Fig 1. At days 6 and 12, DC subsets were recovered and were incubated at 4°C for 15 minutes with 5 μg/mL FITC-coupled streptavidin and 100 μg/mL mouse IgG1 MoAb anti-FITC. (A) Day 6, (B) day 12, and (C) day-12 CD14-derived cells were first incubated for 15 minutes with 50 μg/mL of a blocking MoAb against CD32 followed by 15 minutes with 5 μg/mL FITC-coupled streptavidin and 100 μg/mL mouse IgG1 MoAb anti-FITC. White histograms show FITC streptavidin alone. FITC-coupled isotype match controls were superposable to the white histograms.