Only CD14-derived DCs display nonspecific esterase activity. The CD1a- (A, B, C, D, and E) and CD14- (F, G, H, I, and J) derived DC subsets were obtained as described in Materials and Methods and in the legend to Fig 1. Cells were processed at day 6 (A and F ), day 8 (B and G), day 10 (C and H), day 13 (D and I), and day 16 (E and J) for nonspecific esterase staining. Original magnification × 400. Results are representative of three experiments.