(Left) 3.5% to 17% exponential gradient SDS-polyacrylamide gel of total membrane proteins stained with Coomassie blue, showing a reduction of spectrin content in the patient (lane 2) compared with the control (lane 1). (Upper right) Blood smear of the propositus showing regular numbers of spherocytes and acanthocytes. (Lower right) Family pedigree showing all affected members from two generations. Splenectomized individuals are indicated by asterix and the proband is indicated by an arrow.