Analysis of surface markers by FACS, expression of mRNA transcripts by RT-PCR, and staining for HIV-1 p24 antigen in purified blood DC. (A) Blood DC stained with (a) anti–HLA-DR, (b) anti-CD14, and (c) anti-CD3. (B) TcR and FcγRI (CD64) mRNA expression in DC, T cells, and monocytes. RT-PCR products were analyzed on a 2% agarose gel: TcR (386 bp), CD64 (345 bp), and actin (665 bp) are indicated. Molecular weight markers are on the far left, with their size in basepairs indicated on the left. (C) Immunofluorescence staining of HIV-1–infected blood DC with antibodies against HLA-DR and p24 antigen. Cells were double-stained with FITC-conjugated anti–HLA-DR MoAb (a), an anti-p24 mouse IgG1 MoAb followed by phycoerythrin (PE)-conjugated rat antimouse antibody (b), and an irrelevant, isotype-specific IgG1 control MoAb followed by PE-conjugated rat antimouse antibody (c). Original magnification × 20.