Fig. 4.
Detection of chymase and tryptase in HCMCs by electron microscopic immunocytochemistry. Electron microscopic immunocytochemistry of tryptase and chymase was performed on HCMCs cultured with or without IL-4 (10 ng/mL) for 28 days in the presence of SCF (100 ng/mL) and IL-6 (80 ng/mL). The mast cell cultured without IL-4 stained with antitryptase (a) shows the same reactivity as the mast cell cultured with IL-4 (b). In contrast, the mast cell cultured without IL-4 stained with antichymase (c) shows weak reactivity compared with the mast cell cultured with IL-4 (d). Immunocytochemical control treated with mouse IgG1 instead of antitryptase or antichymase is shown (e). Representative results are shown. (Original magnification × 30,000.)
