Fig. 4.
Fig. 4. Representative results from two to five independent experiments of RT-PCR analysis showing expression of HOXC4, HOXC5, and HOXC6 in normal lymphoid tissues (lanes 1 through 4), T-NHL (lanes 5 through 11), and B-NHL (lanes 12 through 17). MF samples correspond with cases no. 1 and 4, pleomorphic T-cell lymphomas correspond with case no. 12, and ALCLs correspond with cases no. 39, 26, 30, and 32 of Table 1. B-NHLs correspond with cases no. 5, 19, 22, 35, 32, and 41 of Table 2. Controls included B-cell line JVM-3 and water (blanco). The snRNP gene U1A was used as control for the RNA quality and quantity. Blots hybridized with specific oligomer probes were exposed for 20 hours. Note that the immunocytoma in lane 16 (case no. 32), which is positive for HOXC5 expression by RISH analysis, shows a stronger signal for HOXC5 by RT-PCR than the RISH HOXC5-negative immunocytoma in lane 15 (case no. 35).

Representative results from two to five independent experiments of RT-PCR analysis showing expression of HOXC4, HOXC5, and HOXC6 in normal lymphoid tissues (lanes 1 through 4), T-NHL (lanes 5 through 11), and B-NHL (lanes 12 through 17). MF samples correspond with cases no. 1 and 4, pleomorphic T-cell lymphomas correspond with case no. 12, and ALCLs correspond with cases no. 39, 26, 30, and 32 of Table 1. B-NHLs correspond with cases no. 5, 19, 22, 35, 32, and 41 of Table 2. Controls included B-cell line JVM-3 and water (blanco). The snRNP gene U1A was used as control for the RNA quality and quantity. Blots hybridized with specific oligomer probes were exposed for 20 hours. Note that the immunocytoma in lane 16 (case no. 32), which is positive for HOXC5 expression by RISH analysis, shows a stronger signal for HOXC5 by RT-PCR than the RISH HOXC5-negative immunocytoma in lane 15 (case no. 35).

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