Sequence analysis of the A2-FVIII337-372 cross-linked product. Results are presented as subtraction chromatograms wherein the yield (in picomoles) for a given residue (except the initial residue) has subtracted from it the value for that residue in the previous cycle. This method corrects for background and is useful in analyzing low yield samples. The bar graph shows the residue yield for each cycle for experiment no. 1 () and experiment no. 2 (▪). (A) shows the residue yields attributed to the FVIII337-372 peptide sequence and (B) shows the residue yields attributed to the A2 subunit sequence. For (A), the peptide sequence for 10 cycles, using the single letter code, corresponds to MKNNEEAEDY; for (B), the A2 sequence is SVAKKHPKT(W). Trp (W), is typically recovered in low yield, and this residue was not identified at cycle 10 of the A2 sequence.
