Fig. 7.
![Fig. 7. Conditioning regimes prime macrophages to secrete TNFα after LPS stimulation. Peritoneal macrophages were pooled from a minimum of 4 B6D2F1 animals in each group ([▪] naive; [□] 900cGy TBI; [▧] 1,300 cGy TBI) within 4 hours of the completion of TBI and stimulated with LPS (0.1 μg/mL). TNFα was determined by ELISA in the culture supernatants taken 4 hours after stimulation. Data represent the mean ± standard error of quadruplicate wells standardized for 105 macrophages by FACS analysis using F4/80 positivity. Data represent one of three similar experiments. *P < .03 v naive and 1,300 cGy TBI groups.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/8/10.1182_blood.v90.8.3204/4/bl_0004f7.jpeg?Expires=1724275638&Signature=ZeJDSQzhQtT8ZVRavwIc1u2lbVtxTLT70phQAUhlXZzxfWQ2MhC8KHhqINvfiI-cgzayhRnM~DNmW~m0cfoyd-E96xEGZ8D6tHdqO0huhmUcw94QXAVy2IHkCK-P644JyJ84GzDXcGFRWGMeg9-H5eHsTx67X3R9IhGI2LDYLz0gHp5v-U6lv-LVSH6bVhP87tfdxFZHunMPjFmPeze4I7p4TqVtJ2I4w-I-QwDaB9gBz2aIfpnS0CIpAyRwOKTf-p4ufVmgpao~ZcOCYhn0x6j0272dllqdQJwtVcxq8FqeTsa~3IpyB75HEad3U1wNQd2~FuTTfE6M8yxPM~KHpg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Conditioning regimes prime macrophages to secrete TNFα after LPS stimulation. Peritoneal macrophages were pooled from a minimum of 4 B6D2F1 animals in each group ([▪] naive; [□] 900cGy TBI; [▧] 1,300 cGy TBI) within 4 hours of the completion of TBI and stimulated with LPS (0.1 μg/mL). TNFα was determined by ELISA in the culture supernatants taken 4 hours after stimulation. Data represent the mean ± standard error of quadruplicate wells standardized for 105 macrophages by FACS analysis using F4/80 positivity. Data represent one of three similar experiments. *P < .03 v naive and 1,300 cGy TBI groups.
