Western blot analysis of NB4 cells modulated by synthetic retinoids with anti-TM and anti-TF antibodies. NB4 cell lysates (5 × 106 cells) were subjected to immunoblotting analysis using a monoclonal anti-TM antibody, KA-4 (A), and a monoclonal anti-TF antibody, 5G9 (B). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was performed under nonreducing conditions. NB4 cells were incubated with 0.01 μmol/L Am80 (lane 2) or Ro25-7386 (lane 4) for 24 hours. Selective counteractive effect of Ro41-5253 (1 μmol/L) on the effect of Am80 was also observed (lane 3). Lane 1 shows untreated NB4 cell lysate. Molecular weight markers are given along the left margin. Soluble recombinant TM (A; lane 5) and placenta TF (B; lane 5) were used as controls.
