Fig. 2.
Fig. 2. IL1B promoter activity induced by HTLV-I Tax requires two binding sites for NF-IL6 and Spi-1. (A) The scheme shows a representation of the IL1B promoter HT fragment. Specific nucleotide substitutions contained in the two distinct mutated HT fragments are indicated by an arrow. These mutations are located at specific sites known to be critical for NF-IL6 and Spi-1 binding respectively.3234 (B) pcTax, a Tax expression vector (4 μg) was transfected as indicated along with either wild-type or mutated 3MEHT CAT reporters (10 μg). Following transfection, cells were treated with 100 ng/mL of LPS or left untreated. The CAT data were normalized to the average activity elicited by LPS-induced 3MEHT construct in the absence of expression vector cotransfection. Error bars represent SD from three independent experiments. The total amount of transfected DNA was kept constant (14 μg) by the addition of control vector.

IL1B promoter activity induced by HTLV-I Tax requires two binding sites for NF-IL6 and Spi-1. (A) The scheme shows a representation of the IL1B promoter HT fragment. Specific nucleotide substitutions contained in the two distinct mutated HT fragments are indicated by an arrow. These mutations are located at specific sites known to be critical for NF-IL6 and Spi-1 binding respectively.32,34 (B) pcTax, a Tax expression vector (4 μg) was transfected as indicated along with either wild-type or mutated 3MEHT CAT reporters (10 μg). Following transfection, cells were treated with 100 ng/mL of LPS or left untreated. The CAT data were normalized to the average activity elicited by LPS-induced 3MEHT construct in the absence of expression vector cotransfection. Error bars represent SD from three independent experiments. The total amount of transfected DNA was kept constant (14 μg) by the addition of control vector.

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