Effects of cell culture temperature on susceptibility of JKB p16MT cells to apoptosis triggered by dexamethasone. JKB control (▪) and JKB p16MT (□) cells were cultured for 1 day at 40°C; JKB control (▨) or JKB p16MT (▤) were also cultured for 1 day at 31°C. Cells were then cultured for 1 day with Dex (10 μmol/L) at either temperature to induce apoptosis. The percentage of apoptotic cells was determined by acridine orange/ethidium bromide staining (A) and DNA fragmentation (B).