Effects of cell culture temperature on viable cell number, morphology, and cell surface phenotype of JKB p16MT cells. (A) JKB p16MT cells were cultured under the following conditions: 40°C for 7 days (□); 31°C for 7 days (▪); 31°C for 1 day, then 40°C for 6 days (○); 31°C for 2 days, then 40°C for 5 days (⊞); 31°C for 3 days, then 40°C for 4 days (×); and 31°C for 4 days, then 40°C for 3 days, ( —  ▴  — ). JKB control cells were cultured at either 40°C (▵) or 31°C (•) for 7 days. Viable cell number was assessed using trypan blue staining. (B) JKB control (left) and JKB p16MT cells (right) were cultured at 40°C (upper) or 31°C (lower) for 7 days. Cell cytosmears were stained with Wright-Giemsa solution.

(C) JKB control (left) and JKB p16MT (right) cells were cultured at 40°C (upper) or 31°C (lower) for 7 days. Cell surface phenotype was determined by flow cytometric analysis.