Effect of PTX on the PMA-induced VEGF secretion in adherent neutrophils. Neutrophils (10⁷/mL) were incubated (▤) without or with (▪) 100 or (▥) 500 μmol/L of PTX for the indicated periods of times at 37°C with 50 ng/mL of PMA for the indicated period of times at 37°C. Secreted VEGF was assessed by a specific ELISA. Data are from a representative experiment.