American Society of Hematology

Fig. 6.

$Fig. 6. Platelet lysate RT preferentially uses an RNA template. RT assay of sucrose fraction 17 from patient UPN08. Lanes are (from left to right) M, marker; 1 through 5, 2.0, 0.2, 0.02, 0.002, and 0.0002 U of Moloney murine leukemia virus reverse transcriptase; 6 through 13, 1.0 μL of sucrose fraction in duplicate with activated DNA added as follows: lanes 6 and 7, 10 μg; 8 and 9, 1 μg; 10 and 11, 0.1 μg; and 12 and 13, no activated DNA added. The arrow indicates the signal from the RT. Note that the activated DNA can be seen at the top of the gel at the higher concentrations.$

Platelet lysate RT preferentially uses an RNA template. RT assay of sucrose fraction 17 from patient UPN08. Lanes are (from left to right) M, marker; 1 through 5, 2.0, 0.2, 0.02, 0.002, and 0.0002 U of Moloney murine leukemia virus reverse transcriptase; 6 through 13, 1.0 μL of sucrose fraction in duplicate with activated DNA added as follows: lanes 6 and 7, 10 μg; 8 and 9, 1 μg; 10 and 11, 0.1 μg; and 12 and 13, no activated DNA added. The arrow indicates the signal from the RT. Note that the activated DNA can be seen at the top of the gel at the higher concentrations.

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