Detection of the HLA-H Cys282Tyr and His63Asp mutations by PCR-RFLP. The top panel shows the result of PCR with the codon 282 primers and restriction digest by Rsa I. The bottom panel is PCR product obtained with the codon 63 primers and digested with Bcl I. The patients are as follows: track 1, normal; track 2, homozygous mutant, codon 282; normal, codon 63; track 3, heterozygous mutant, codon 282; normal codon 63; track 4, compound heterozygote; track 5, water negative control.