Direct DNA sequencing of the FGFR3 mutation in the KMS-11 cell line. The 3′ primer used for the amplification of the TM-containing fragments of the FGFR3 gene was used as sequencing primer for both the DNA (164 bp) and cDNA (204 bp) templates (see Text). The normal sequence across codon 373 is reported. The base pair mutation is indicated by an arrow. DNA from normal peripheral blood leukocytes was used as a control (Normal).