Cross-blocking experiments between CAGAS and mouse MoAb CSLEX-1 (anti-sLex) for their binding to PMN leukocytes (PMN) and between CAGAS and mouse MoAbs CSLEX-1 and 19.9 (anti-sLa) for their binding to COLO205 cells (COLO205). Histograms show the binding of a subsaturating dose of biotinylated (Bio) antibody (indicated at the top of histograms) in the presence of PBS or an excess of unconjugated antibody (indicated within each histogram). For control purposes, mouse MoAb CD45 (138-3) of IgM isotype-like CSLEX-1 was also included as inhibitor. The binding of antibodies was detected by means of SA-PE. Negative control fluorescence curves are the most proximal to the Y-axis and were Bio-MPoly for Bio-GAS and biotinylated isotype-matched unreactive mouse for Bio-CSLEX-1 and Bio-19.9.
