American Society of Hematology

Fig. 3.

$Fig. 3. Detection of partial DJH rearrangements in BM cell fractions. DNA from cell lysates was amplified with a mixture of consensus primers for most of DH gene families and a consensus JH primer. PCR products were visualized by Southern blot hybridization using an internal consensus JH probe. GL DHQ52 indicates the amplification, from a germline IgH locus, of the DNA segment located between the most 3′ DH gene (DHQ52 ) and the most 5′ JH gene (JH1 ), producing a 160-bp product.$

Detection of partial DJ_H rearrangements in BM cell fractions. DNA from cell lysates was amplified with a mixture of consensus primers for most of D_H gene families and a consensus J_H primer. PCR products were visualized by Southern blot hybridization using an internal consensus J_H probe. GL D_HQ52 indicates the amplification, from a germline IgH locus, of the DNA segment located between the most 3′ D_H gene (D_HQ52 ) and the most 5′ J_H gene (J_H1 ), producing a 160-bp product.

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