American Society of Hematology

Fig. 2.

$Fig. 2. Detection of VDJH rearrangements in BM cell fractions. DNA isolated from purified cell fractions (A) or dilutions of CD34− CD19+ B cells in keratinocytes (B) were amplified with consensus FR3 and JH primers. PCR products were subjected to Southern blot hybridization using an internal consensus JH oligonucleotide probe. Amplification of the RAG-1 gene served as control for DNA integrity.$

Detection of VDJ_H rearrangements in BM cell fractions. DNA isolated from purified cell fractions (A) or dilutions of CD34⁻ CD19⁺ B cells in keratinocytes (B) were amplified with consensus FR3 and J_H primers. PCR products were subjected to Southern blot hybridization using an internal consensus J_H oligonucleotide probe. Amplification of the RAG-1 gene served as control for DNA integrity.

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