Fig. 2.
Competitive PCR performed with 10−1 dilution of AML 679 (upper panel) and 100 dilution of CD34a (lower panel), containing equal amounts of cDNA as verified by β-actin PCR. In both PCRs, dilutions (10−1 per step) starting from 1 pg standard cDNA were performed and coamplified with identical amounts of sample cDNA as used for the experiment shown in Fig 1. The wt1 cDNA amount in AML 679 10−1 was 1.5 fg; in CD34a 100, 2 fg was determined. Cst, standard control; Cspl, sample control.
