Fig. 1.
Expression of β-actin and wt1 genes in 5 representative AML blast samples and 2 CD34+ normal progenitor populations. Amplification of the cDNA derived from AML blasts was performed with 100 (left), 10−1 (medium), and 10−2 (right) dilutions. cDNA derived from normal cells was diluted 100 (left) and 10−1 (right) due to the lower cell number obtaind for RNA extraction. Dilutions were performed to avoid PCR saturation due to initial excess of template.