(A) Southern blot analysis of amplified vector sequences from baboon, J94090. Primers LN/LNL6 2229 and LN/LNL6 3210 were used to distinguish signals derived from LN (301 bp) versus LNL6 (982 bp). PCR for actin DNA was performed on the same samples with 100 ng DNA. Standards consist of single copy HT1080 DNA, HT1080/LN, and HT1080/LNL6 at a 1:1 ratio, mixed with normal baboon DNA in a log dilution series. PB, peripheral blood; BM, bone marrow. (B) Percentage vector-positive cells measured by phosphorimage analysis of signal intensities for LN and LNL6 corrected for the LN/LNL6 ratio in the standards (see Results) and for the amount of DNA as determined by actin PCR. (C) Southern blot analysis of neo-amplified sequences from PB, BM, peripheral blood granulocytes (Gran), and CD2⁺ T lymphocytes (using neo primers neo 350 and neo 1150). (D) LN/LNL6 ratios over time in PB cells of the long-term surviving animal, J94090.