Fig. 5.
![Fig. 5. Induction of endogenous GAS-binding activity by Bmx expression. COS cells were transfected with Bmx, Jak2, or control expression vectors, as shown, and nuclear extracts of transfected cells were prepared. Endogenous Stat activation was analyzed in the mobility shift assay using 32P-labeled IRF-1 GAS oligonucleotide. The same lysates were also incubated with anti-Stat1 antibody (ISGF3 [p91]) before mobility shift analysis with the IRF-1 GAS probe. As a control we analyzed a COS lysate containing overexpressed Stat1 and Bmx.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/11/10.1182_blood.v90.11.4341/3/bl_0014f5.jpeg?Expires=1724242605&Signature=pCKUWBfOplphOuQ~FZwHqZzr4QgM3L9f0Dbexaa~1wWwwAq~OnJoGuhYhhqGlfSRfrJ3i2LICd36Op0Tmvu~ZuoqgE4Tn90Sj9V1CANqV~aq6XW071Onh66lGoUZkoiHdMupSOzOyNWB5wSKGgwsqqagziCUl8nE1SW6p4KcuiAO8p5EqD3hjzO-vGURUzLsHlhwxPU2kF8-FcsbRL7iGnzmbfKOepGRT-VO6I89xQdeWD8pdmze4HkG1PsOQMSiYS~8vShffMgsWNO1vMLmH9QmpDaWqzCxJ7EH-CortylkC87U4KK0QxWCTlrjJ8mkFTkzj-1NiavNPpLemxPbyA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Induction of endogenous GAS-binding activity by Bmx expression. COS cells were transfected with Bmx, Jak2, or control expression vectors, as shown, and nuclear extracts of transfected cells were prepared. Endogenous Stat activation was analyzed in the mobility shift assay using 32P-labeled IRF-1 GAS oligonucleotide. The same lysates were also incubated with anti-Stat1 antibody (ISGF3 [p91]) before mobility shift analysis with the IRF-1 GAS probe. As a control we analyzed a COS lysate containing overexpressed Stat1 and Bmx.
