Fig. 5.
![Fig. 5. Platelet aggregation and Ca++ influx. Washed platelets were prepared as described under Materials and Methods. For intracellular Ca++ uptake (Δ[Ca2+]i) detection, platelets were loaded with the photoprotein aequorin. MgCl2 (1.0 mmol/L), CaCl2 (1.0 mmol/L), and fibrinogen (final concentration: 800 μg/mL) were added before the experiments. Aggregation and luminescence following incubation with transfected COS-7 cells were recorded simultaneously. Each box displays one representative experiment for COS-7 to platelet ratios of 1:280. The upper (+)-tagged lines show human platelet aggregation as percentage of light transmission. The lower (×)-tagged lines give the simultaneously recorded change in luminescence due to platelet Δ[Ca2+]i .](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/11/10.1182_blood.v90.11.4425/3/bl_0013f5.jpeg?Expires=1724236139&Signature=TkjWn7IMnzENhUDPC9bffJ0m8PtWAJkUmLBiIneNJ7ICeDFvoZ5sJ4Q59etTixEOxzp3z~g62BDAAQC6i-e8jiSpcPqRFF2ZfE577IX4tw3w71UQnO8FGWe-iej6X96vSbs34lHJxs1swyp-kV1FNeJNCXnCM~RDYwW7B6eRmtkdovcC8zLaXIHAzPLCSvRxLt9CcnSTPi-cab9AQ1baVpSysg5Skq28I~AXgbBbF2BG9aBk2sBSJvkXMY-p0QmJ-DWVN8gezeb6OsMJK6eSUNp1qaMJOMQHto7qqxIXBDdIJT5QoqHIIR00K8Gx4ot4UlMd6TjHFiuXvFITmEEIAA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Platelet aggregation and Ca++ influx. Washed platelets were prepared as described under Materials and Methods. For intracellular Ca++ uptake (Δ[Ca2+]i) detection, platelets were loaded with the photoprotein aequorin. MgCl2 (1.0 mmol/L), CaCl2 (1.0 mmol/L), and fibrinogen (final concentration: 800 μg/mL) were added before the experiments. Aggregation and luminescence following incubation with transfected COS-7 cells were recorded simultaneously. Each box displays one representative experiment for COS-7 to platelet ratios of 1:280. The upper (+)-tagged lines show human platelet aggregation as percentage of light transmission. The lower (×)-tagged lines give the simultaneously recorded change in luminescence due to platelet Δ[Ca2+]i .
