Fig. 6.
Esterase expression by the different MTHC populations. The cell groups are the same as those shown in Fig 5. Red staining is indicative of α-naphthyl acetate esterase and blue is indicative of naphtol-AS-D-chloroacetate esterase, with methyl green as the counterstain. (a and d) Nonadherent and adherent parental MTHC cultured in murine GM-CSF, (b and e) nonadherent and adherent WTKit-expressing MTHC maintained in human SCF, and (c and f ) nonadherent and adherent V816Kit-expressing MTHC maintained without factor. The bar in (a) is equivalent to 38 μm and all photographs are at the same magnification.

Esterase expression by the different MTHC populations. The cell groups are the same as those shown in Fig 5. Red staining is indicative of α-naphthyl acetate esterase and blue is indicative of naphtol-AS-D-chloroacetate esterase, with methyl green as the counterstain. (a and d) Nonadherent and adherent parental MTHC cultured in murine GM-CSF, (b and e) nonadherent and adherent WTKit-expressing MTHC maintained in human SCF, and (c and f ) nonadherent and adherent V816Kit-expressing MTHC maintained without factor. The bar in (a) is equivalent to 38 μm and all photographs are at the same magnification.

Close Modal

or Create an Account

Close Modal
Close Modal