LasBD restores normal integrin function and expression. (A) MO7e, MO7e^p210, and LasBD-MO7e^p210 bulk-selected and cloned cells were stained with FITC- or PE-conjugated monoclonal antibodies against CD29 or CD44 and analyzed on a FACStar^PLUS flow cytometer. PE- and FITC-conjugated isotype matched mouse IgGs were used as negative controls. Results are presented as the mean ± SEM of four individual experiments. Statistics: Paired Student's t-test: P < .001. (B) Aliquots of 5 to 10 × 10⁴ normal CD34⁺ cells, primary CML CD34⁺ HLA-DR⁺ cells or MO7e, MO7e^p210 cells and LasBD-MO7e^p210 bulk-selected cells were incubated with an adhesion blocking anti-β1 antibody followed by a goat-antimouse FITC antibody, as described in the Materials and Methods. Cytospin preparations were examined by immunofluorescence microscopy for the presence or absence of integrin caps.