Fig. 8.
Fig. 8. Effects of jararaca GPIb-BP or GRGDS peptide plus EGTA on the cytoskeletal association of p60c-src and p54/58lyn induced by botrocetin-vWF. Platelets were incubated with or without 10 μg/mL of jararaca GPIb-BP (A and B) or with or without 200 μmol/L GRGDS peptide plus 1 mmol/L EGTA (instead of Ca2+) (C and D) for 5 minutes and then activated with 3 μg/mL of botrocetin and 10 μg/mL of vWF for the indicated time intervals. Cytoskeletal fractions were harvested as described in the legend for Fig 7. Immunoblot analysis of cytoskeletal fractions were performed with anti-p60c-src (A and C) and anti-p54/58lyn (B and D) MoAbs.

Effects of jararaca GPIb-BP or GRGDS peptide plus EGTA on the cytoskeletal association of p60c-src and p54/58lyn induced by botrocetin-vWF. Platelets were incubated with or without 10 μg/mL of jararaca GPIb-BP (A and B) or with or without 200 μmol/L GRGDS peptide plus 1 mmol/L EGTA (instead of Ca2+) (C and D) for 5 minutes and then activated with 3 μg/mL of botrocetin and 10 μg/mL of vWF for the indicated time intervals. Cytoskeletal fractions were harvested as described in the legend for Fig 7. Immunoblot analysis of cytoskeletal fractions were performed with anti-p60c-src (A and C) and anti-p54/58lyn (B and D) MoAbs.

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