American Society of Hematology

Fig. 7.

$Fig. 7. Cytoskeletal association of p60c-src, p54/58lyn, and p72syk induced by botrocetin-vWF. Platelets were activated with 3 μg/mL of botrocetin and 10 μg/mL of vWF for the indicated time intervals. Reactions were then stopped with lysis buffer, and Triton-X–insoluble fractions were harvested by centrifugation at 16,000g for 5 minutes. The pellets were solubilized with Laemmli buffer. Cytoskeletal association of tyrosine kinases is detected by Western blotting using anti-p60c-src (A), anti-p54/58lyn (B), and anti-p72syk (C) MoAbs. w, whole cell lysates.$

Cytoskeletal association of p60^c-src, p54/58^lyn, and p72^syk induced by botrocetin-vWF. Platelets were activated with 3 μg/mL of botrocetin and 10 μg/mL of vWF for the indicated time intervals. Reactions were then stopped with lysis buffer, and Triton-X–insoluble fractions were harvested by centrifugation at 16,000g for 5 minutes. The pellets were solubilized with Laemmli buffer. Cytoskeletal association of tyrosine kinases is detected by Western blotting using anti-p60^c-src (A), anti-p54/58^lyn (B), and anti-p72^syk (C) MoAbs. w, whole cell lysates.

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