American Society of Hematology

Fig. 2.

$Fig. 2. Serial high-resolution cell-cycle analysis of cytokine-stimulated G0 and G1 CD34+ cells. MPB G0 and G1 CD34+ cells were plated in complete medium supplemented with IL-3-IL-6-SCF. At indicated times, cells were harvested and analyzed for cell-cycle status with 7-AAD and Ki-67. (A) Dual-parameter plot showing fractionation of CD34+ cells in G0 and G1 phases with Hst and PY. (B to D) Cell-cycle analysis of cells harvested from G1 CD34+ cell–initiated culture at 24, 72, and 144 hours, respectively. (E to G) Cell-cycle analysis of cells harvested from G0CD34+ cell–initiated culture at 24, 72, and 144 hours, respectively. The percentage of cells in G0 , G1 , and S/G2 + M (abbreviated as G2 ) is indicated. Cells in the lower right quadrant were not considered.$

Serial high-resolution cell-cycle analysis of cytokine-stimulated G₀ and G₁ CD34⁺ cells. MPB G₀ and G₁ CD34⁺ cells were plated in complete medium supplemented with IL-3-IL-6-SCF. At indicated times, cells were harvested and analyzed for cell-cycle status with 7-AAD and Ki-67. (A) Dual-parameter plot showing fractionation of CD34⁺ cells in G₀ and G₁ phases with Hst and PY. (B to D) Cell-cycle analysis of cells harvested from G₁ CD34⁺ cell–initiated culture at 24, 72, and 144 hours, respectively. (E to G) Cell-cycle analysis of cells harvested from G₀CD34⁺ cell–initiated culture at 24, 72, and 144 hours, respectively. The percentage of cells in G₀ , G₁ , and S/G₂ + M (abbreviated as G₂ ) is indicated. Cells in the lower right quadrant were not considered.

This Feature Is Available To Subscribers Only