American Society of Hematology

Fig. 1.

$Fig. 1. General method of cell-cycle fractionation using DNA and RNA analysis with Hst and PY. (A) Dual-parameter dot plot showing analysis of BM CD34+ cells stained with Hst and PY. Cells residing in G0 (low PY uptake) appear at the bottom of the G0 /G1 region identified by low Hst staining. Cells in G1 have a brighter PY signal within the G0 /G1 peak. Cells in S/G2 + M display both high Hst and high PY staining. (B) Postsort analysis of BM CD34+ cells sorted in G0 . (C) Hst/PY fluorescence distribution of G0 CD34+ cells shown in B after 24 hours under IL-3-IL-6-SCF stimulation, showing G0-G1 progression. (D) Same sample after 72 hours in culture, depicting cells moving into S/G2 + M phase.$

General method of cell-cycle fractionation using DNA and RNA analysis with Hst and PY. (A) Dual-parameter dot plot showing analysis of BM CD34⁺ cells stained with Hst and PY. Cells residing in G₀ (low PY uptake) appear at the bottom of the G₀ /G₁ region identified by low Hst staining. Cells in G₁ have a brighter PY signal within the G₀ /G₁ peak. Cells in S/G₂ + M display both high Hst and high PY staining. (B) Postsort analysis of BM CD34⁺ cells sorted in G₀ . (C) Hst/PY fluorescence distribution of G₀ CD34⁺ cells shown in B after 24 hours under IL-3-IL-6-SCF stimulation, showing G₀-G₁ progression. (D) Same sample after 72 hours in culture, depicting cells moving into S/G₂ + M phase.

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