American Society of Hematology

Fig. 3.

$Fig. 3. Staining of FBM cells with AC133. Ammonium chloride–lysed FBM cells were labeled with AC133 and goat anti-mouse PE, followed by staining with CD34FITC (A through E) and CD38APC and CD50PerCP (B), or CD19APC (C), or CD64APC and CD71PerCP (D and E). (A) Unfractionated FBM cells. (B through E) Staining of CD34+ cells only, gated from region R1 in A. Noncommitted progenitor cells (CD34hiCD38loCD50+ ) are represented by enlarged violet dots in B, and lymphoid committed progenitors (CD34+CD19+ ) are depicted in blue in C. Granulomonocytic committed progenitors (CD34+CD64+ ) are represented by green dots in D, and erythroid committed progenitors (CD34hiCD71hiCD64− ) are depicted in red in E. Dashed lines represent isotype control levels for each of the colored populations. tOLS, transformed orthogonal light scatter. The results are representative of 3 experiments.$

Staining of FBM cells with AC133. Ammonium chloride–lysed FBM cells were labeled with AC133 and goat anti-mouse PE, followed by staining with CD34FITC (A through E) and CD38APC and CD50PerCP (B), or CD19APC (C), or CD64APC and CD71PerCP (D and E). (A) Unfractionated FBM cells. (B through E) Staining of CD34⁺ cells only, gated from region R1 in A. Noncommitted progenitor cells (CD34^hiCD38^loCD50⁺ ) are represented by enlarged violet dots in B, and lymphoid committed progenitors (CD34⁺CD19⁺ ) are depicted in blue in C. Granulomonocytic committed progenitors (CD34⁺CD64⁺ ) are represented by green dots in D, and erythroid committed progenitors (CD34^hiCD71^hiCD64⁻ ) are depicted in red in E. Dashed lines represent isotype control levels for each of the colored populations. tOLS, transformed orthogonal light scatter. The results are representative of 3 experiments.

This Feature Is Available To Subscribers Only