Anti–E- and anti–P-selectin antibodies do not influence the development of EAE. Inflammatory cuffs in the CNS of mice afflicted with EAE are shown. Left panel: staining of vessels with anti-PECAM-1/CD31; right panel: staining of leukocytes with anti-CD45. Inflammatory cuffs develop in rat IgG-treated (control) EAE animals but also in presence of anti–E- or anti–P-selectin or both MoAbs. In contrast, in animals treated with anti–α4-integrin, MoAb inflammatory cuffs cannot be detected. Single CD45⁺ cells adjacent to the vessels can also be seen in the healthy CNS and are most probably perivascular macrophages. Immunoperoxidase staining, hematoxylin counterstain. Original magnification × 200.