Parallel TGGE. Same mtDNA fragment as in Fig 7. After denaturation and renaturation, each PCR product was brought onto the gel in triplicate at time intervals of 15 minutes (lanes a, b, and c, respectively). The running time was too long for the samples designated a and b, so that bands became diffuse. Clear bands can be seen in lanes c. M, D1, and D2, normal bands in the mother and two daughters of patient no. 1, respectively; T and Mu, normal bands in T lymphocytes and buccal mucosa cells of patient no. 1, respectively; P1 and P2, heteroduplex bands in patients no. 1 and 2, respectively; Co, normal bands in an additional control person.