Fig. 5.
Analysis of rAAV-LacZ genome in high molecular weight DNA from developing T cells derived from CD34+CD2− isolated at various time points after culture on thymic stroma. Shown are representative data from five experiments at either early time points (14 to 21 days) or later time points (28 to 42 days). PCR was performed with LacZ-specific primers and the identity of the products was confirmed by Southern blotting with a digoxygenin-labeled probe specific to an internal region of LacZ. Gsα primers were used as a positive control for genomic DNA quality and a single 494-bp product was detected.
