CP-10 protein from control, LPS-activated, or IL-1–activated EC lysates. CP-10 was enriched from lysates of control (lane 2), IL-1–activated (lane 3), and LPS-activated (lane 4) bEnd-3 cells by anti–CP-10 polyclonal antibody affinity chromatography. Eluted protein was examined by Western blot analysis as described in the Materials and Methods. Similar results were obtained in three experiments. Recombinant CP-10 (lane 1) was used as a positive control and the positions of molecular weight standards are shown.