(A and B) Dose-response of CP-10 mRNA induction in bEnd-3 cells cultured for 24 hours with LPS or IL-1β. Insets show Northern blots hybridized with CP-10 and 18S probes. Quantitation densitometry of CP-10 mRNA is given. (C) Northern blot analysis of bEnd-3 cells stimulated for 24 hours as follows: lane 1, control, unstimulated; lane 2, LPS (100 ng/mL); lane 3, LPS and control IgG (0.5 μg/mL); lane 4, LPS and neutralizing anti-TNFα MoAb (0.5 μg/mL); and lane 5, LPS and IL-1RA (100 ng/mL). The blot was rehybridized with a rat 18S RNA probe. Results are representative of two experiments.