Northern blot analysis of CP-10 and MRP-14 mRNA expression by bEnd-3 cells after 24 hours of stimulation. (A) Control unstimulated, IL-1β (340 U/mL), LPS (100 ng/mL), TNFα (12 U/mL), IFNγ (100 U/mL), and IL-1α (150 U/mL). (B) After 24 hours of culture with vitamin D3 (100 nmol/L), calcium ionophore 23187 (10 μmol/L), or PMA (10 ng/mL). (C) RNA from murine bone marrow cells (BM) and 24-hour (1) unstimulated or (2) LPS-activated (100 ng/mL) bEnd-3 cells was hybridized with the ³²P-labeled CP-10 hinge region oligonucleotide probe. (D) CP-10 mRNA expression by bEnd-3, tEnd-1, and sEnd-1 cells cultured alone (1) or with LPS (100 ng/mL) for 24 hours. Membranes from (A) and (B) were rehybridized with a rat 18S RNA probe. Results are representative of at least three experiments.