Pseudosubstrate inhibition of TF-FVIIa on HL-60 cells before and after ionomycin stimulation. The details of these order of addition experiments are presented in Materials and Methods. The data were corrected for cell loss and normalized against a control sample (100% TF PCA) as described. The black bars are the data from the experiment employing TFPI-FXa (N = 3). The gray bars are the data from the XK1 experiment (N = 3). The standard deviations are represented by the error bars. The order of addition groups were as follows: (1) FVIIa → DMSO → buffer → wash → ionomycin (2) FVIIa → ionomycin → buffer → wash → ionomycin (3) FVIIa → DMSO → TFPI-FXa (A) or XK1 (B) → wash → ionomycin (4) FVIIa → ionomycin → TFPI-FXa (A) or XK1 (B) → wash → ionomycin.