Northern analysis of the expression of Exodus. (A) Exodus is not expressed in a variety of hematopoietic and nonhematopoietic cell lines in poly A+ Northern analysis. (B) Commercially prepared poly A+ Northern blots of a variety of normal human lymphoid and nonlymphoid tissue. Exodus is preferentially expressed in lymphoid tissue, consistent with the other members of the β-chemokine family. (C) Exodus expression is induced in monocytic and endothelial cells when they are exposed to inflammatory stimuli. PBMC are normal human PBMCs, I-HUVEC are cultured human umbilical vein endothelial cells, and THP-1 are human monocytic leukemia cells. LPS is lipopolysaccharide. The blots in 2A and 2C were rehybridized with actin to control for integrity and loading of RNA. These signals are shown below the Exodus Northerns. The commercially-prepared Northerns were not rehybridized with actin.