Fig. 5.
![Fig. 5. IL-10 inhibits FL + IL-7–stimulated proB cell proliferation. A total of 500 Lin−Sca-1+c-kit+ cells was seeded in complete IMDM in the presence of FL + IL-7. After 12 days incubation, 1 × 105 of the generated B220+ cells were replated in triplicates in complete IMDM in medium alone, FL + IL-7, or FL + IL-7 + IL-10, as indicated. Cells were incubated for 48 hours and pulsed with 1 μCi [3H]-TdR during the last 12 hours of incubation. Samples were harvested and counted in a beta-counter as described in the Materials and Methods. Results represent the mean cpm (SEM) from three independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/11/10.1182_blood.v90.11.4321/3/bl_0029f5.jpeg?Expires=1722713436&Signature=GMwINCEgUHuuIUonc6GTp818uRwSpt9HkkyYnImRJnbzW9dcBJKDhizj9of3m7STG09qrml1m4vu00QNJigBarT393EbzAkDOhjXD8SfR-LpNjPojm5HPoAxACaK-bW5XC9ckJU70zdMOb0zj1fxdk4lYoq9akUfmkGf74PQ6~-OujNMRX~PQvs-Ig~r-~b8sOI5wiVlL2fERBwTRAk2hrTSOf~RBFD7YL37WxSHcPfuZtJ6ryMeZ25LrSrDJGZRN~UTryr5~rWo3TvblvbDHEj9RzvvWTeRVkBVjTBajmwpG~zeuPMhkrTPHWsNHSspKaCoL4Zx570yo9~zy~AlJw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IL-10 inhibits FL + IL-7–stimulated proB cell proliferation. A total of 500 Lin−Sca-1+c-kit+ cells was seeded in complete IMDM in the presence of FL + IL-7. After 12 days incubation, 1 × 105 of the generated B220+ cells were replated in triplicates in complete IMDM in medium alone, FL + IL-7, or FL + IL-7 + IL-10, as indicated. Cells were incubated for 48 hours and pulsed with 1 μCi [3H]-TdR during the last 12 hours of incubation. Samples were harvested and counted in a beta-counter as described in the Materials and Methods. Results represent the mean cpm (SEM) from three independent experiments.
