HGF-SF and c-met expression in myeloid and lymphoid cell lines. (A) Northern hybridization for HGF-SF (above) and c-met transcripts (middle) in human myeloid and lymphoid cell lines: embryonic lung fibroblasts (control) (1), KG-1 (2), HL-60 (3), K562 (4), HEL (5), U937 (6), M(ono)M(ac)6 (7), Molt-3 (8), SKW-3 (9), REH (10), JVM-2 (11), MN-60 (12), and OPM-2 (13). Faint bands in lane 2 (KG-1) of c-met hybridization represent a remnant signal from hybridization for HGF-SF. In lanes 10 and 13 (HGF-SF ) and 10 (c-met ) faint signals (arrows) were detectable only after extended exposure times (see also Table 2). Rehybridization for GAPDH is shown below. (B) HGF-SF expression in cell line M(ono)M(ac)6 without (MM6) and after stimulation with dimethylsulfoxide (MM6 + DMSO), cyclic AMP (MM6 + cAMP), and phorbol ester (MM6 + TPA). (C) c-met expression in cell line K562 without (K562) and after stimulation with DMSO (K562 + DMSO), dbcAMP (K562 + cAMP) and TPA (K562 + TPA). Rehybridizations for GAPDH in (B) and (C) are shown below.