Fig. 2.
G-CSF deprivation results in loss of the B subunit. Neutrophils were disrupted by sonication either immediately (Immed) or after cultivation for 60 minutes in the presence (G-CSF ) or absence (Control) of G-CSF. Samples representing cytosol (100,000g supernatant) of 2 × 107cell equivalents were resolved by SDS-PAGE and detected with anti-B2 antibody.

G-CSF deprivation results in loss of the B subunit. Neutrophils were disrupted by sonication either immediately (Immed) or after cultivation for 60 minutes in the presence (G-CSF ) or absence (Control) of G-CSF. Samples representing cytosol (100,000g supernatant) of 2 × 107cell equivalents were resolved by SDS-PAGE and detected with anti-B2 antibody.

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