Fig. 2.
Fig. 2. Sac I digestion of PCR-amplified exon 18. The G to A substitution at nt 2354 results in the creation of a unique Sac I restriction site. The digestion pattern displays a 164-bp fragment for normal amplified fragment and two fragments of 116 and 48 bp for the mutated amplified fragment. M, HaeIII digest of ØX-174-RF DNA molecular weight marker.

Sac I digestion of PCR-amplified exon 18. The G to A substitution at nt 2354 results in the creation of a unique Sac I restriction site. The digestion pattern displays a 164-bp fragment for normal amplified fragment and two fragments of 116 and 48 bp for the mutated amplified fragment. M, HaeIII digest of ØX-174-RF DNA molecular weight marker.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal