CIS-induction by IL-2 in F-7 and H-4 cells. Ba/F3 cells expressing the wild-type IL-2 receptor β chain (F-7) or cells expressing the H-mutant (H-4) were factor-depleted for 4 hours, and stimulated with either IL-2 or IL-3 for the indicated periods (h). Total RNA (5 μg/lane) was separated from the cells and blotted with CIS, c-myc, and α-enolase probes.